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Image Search Results
Journal: Journal of Experimental Neuroscience
Article Title: Epigenetic Mechanisms of the Aging Human Retina
doi: 10.4137/JEN.S25513
Figure Lengend Snippet: Genome-wide epigenetic studies of retinal disease, risk factors, and related pathologies.
Article Snippet: Ko et al, 2013 , Renal , Kidney fibrosis , Discovery: HELP-Nimblegen whole genome covering microarray; validation:
Techniques: Genome Wide, RNA Expression, Preserving, Methylation, Microarray, Immunohistochemistry, Western Blot, Activity Assay, Binding Assay, Methylated DNA Immunoprecipitation, Biomarker Discovery, DNA Methylation Assay, Cell Culture, Extraction, Methylation Sequencing, Transformation Assay, Sequencing, Methylated DNA Immunoprecipitation Sequencing, Phospho-proteomics, Glycoproteomics, Luciferase, Starch, In Vitro, Expressing, Transduction, Ubiquitin Proteomics, Clinical Proteomics, Membrane, Cell Differentiation, Control
Journal: International Journal of Molecular Sciences
Article Title: DNA Demethylation Induces Tree Peony Flowering with a Low Deformity Rate Compared to Gibberellin by Inducing PsFT Expression under Forcing Culture Conditions
doi: 10.3390/ijms23126632
Figure Lengend Snippet: Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on MassARRAY (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).
Article Snippet: Quantitative methylation analysis of bisulfite-treated genomic DNA was conducted through Agena’s
Techniques: DNA Methylation Assay, Expressing, Quantitative RT-PCR, Concentration Assay